pdgf aa elisa kits  (R&D Systems)

Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A The images of HK-2 cells treated with different concentrations of H. pluvialis were captured by an inverted optical microscope ( n = 3). B CCK-8 viability assay evaluating HK-2 cell proliferation under various doses and treatment durations of H. pluvialis ( n = 3). C ELISA quantification of CTGF and PDGF secretion in HK-2 cells ( n = 3). D qRT-PCR analysis of SASP markers in HK-2 cells ( n = 3). ACTB was used as a reference gene. E GSEA signaling enrichment analyses in TGF-β1-stimulated HK-2 cells via RNA-seq. F Functional enrichment analysis of CCN2 gene and SERPINE1 gene via RNA-seq profiling. * p < 0.05 vs. Con group, # p < 0.05 vs. TGF-β1 group. For GSEA interpretation, thresholds were set at |normalized enrichment score (NES)| >1, nominal p < 0.05, and FDR q < 0.5.

Article Snippet: The levels of connective tissue growth factor (CTGF) and platelet-derived growth factor (PDGF) were determined using Human CTGF ELISA Kit (EK198, Multisciences (Lianke) biotech Co., Ltd, Hangzhou, China) and Human PDGF ELISA Kit (EK12318, Multisciences (Lianke) biotech Co., Ltd, Hangzhou, China).

Techniques: Microscopy, CCK-8 Assay, Viability Assay, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, RNA Sequencing, Functional Assay

Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A Blue: Nuclei. Purple: Mitochondrial. Red: PINK1/Parkin/p62/LC3B. IF staining was performed to detect PINK1, Parkin, p62, and LC3B protein levels (magnification: 100×, scale bar: 15 μm). B Colocalization analysis of p62, LC3B, and mitochondria was performed using ImageJ software. C Colocalization Pearson correlation coefficient analysis were obtained based on IF images of p62 and LC3B ( n = 6). D Western blot analysis of PINK1 and LC3B expression in renal cortical tissues from different experimental groups. Quantification of gray values for Western blotting results in ( D ) was performed using ImageJ ( n = 3). E Blue: Nuclei. Red: E-cadherin/N-cadherin/Vimentin. IF assay was performed to detect E-cadherin, N-cadherin and Vimentin protein levels (magnification: 100×, scale bar: 15 μm). F Quantitative analysis of fluorescence intensity using ImageJ software ( n = 6). G ELISA quantification of CTGF and PDGF secretion in HK-2 cells ( n = 3). H Western blot analysis of E-cadherin, N-cadherin and Vimentin expression in TGF-β1-induced HK-2 cells. Quantification of gray values for Western blotting results in ( H ) was performed using ImageJ ( n = 3). I Representative flow cytometry plots showing the detection of MMP. J Quantitative analysis of flow cytometry fluorescence intensity of ROS ( n = 3). * p < 0.05 vs. Con group, # p < 0.05 vs. TGF-β1 group. a p < 0.05 vs. HP group, b p < 0.05 vs. AST group.

Article Snippet: The levels of connective tissue growth factor (CTGF) and platelet-derived growth factor (PDGF) were determined using Human CTGF ELISA Kit (EK198, Multisciences (Lianke) biotech Co., Ltd, Hangzhou, China) and Human PDGF ELISA Kit (EK12318, Multisciences (Lianke) biotech Co., Ltd, Hangzhou, China).

Techniques: Staining, Software, Western Blot, Expressing, Fluorescence, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: Anesthesiology

Article Title: Platelet-derived Growth Factor Receptor-α Induces Contraction Knots and Inflammatory Pain–like Behavior in a Rat Model of Myofascial Trigger Points

doi: 10.1097/ALN.0000000000005167

Figure Lengend Snippet: Receptor tyrosine kinases (RTKs) expression profiles of myofascial trigger points (MTrPs) tissue derived from upper trapezius muscle of myofascial pain syndrome (MPS) patients and control groups. ( A ) The upregulated RTKs members were validated by microarray analysis. MPS group = 11, Con group = 7. Platelet-derived growth factor receptor-α (PDGFR-α): Con, 879 ± 83.08; 95% CI, 802.2–955.8 versus MPS, 1,060 ± 96.84; 95% CI, 994.9–1,125; units, fluorescent value; mean ± SD; P < 0.001. PDGFR-β: Con, 338.8 ± 47.96; 95% CI, 294.5–383.2 versus MPS, 308.4 ± 51.67; 95% CI, 273.7–343.1; units, fluorescent value; mean ± SD; P > 0.05. ( B ) Representative microscopic images showing morphology of muscle fibers in different groups. The morphology of MTrPs showed that annular or enlarged muscle fibers ( yellow arrows ) of different sizes with centralized nuclei in cross-sectional spaces under microscopy ( yellow arrows ). Scale bars, 20 μm. ( C ) The relationship between pain intensity and expression level of phosphorylated PDGFR-α (p-PDGFR-α) was characterized by a significant positive correlation (r = 0.711; n = 11; P < 0.05). ( D ) Results from enzyme-linked immunosorbent assay (ELISA) showed that the level of serum platelet-derived growth factor-AA (PDGF-AA) was increased. Con, 3.74 ± 0.82; 95% CI, 2.96–4.5; versus MPS, 5.97 ± 0.98; 95% CI, 5.31–6.6; units, ng/ml; mean ± SD; P < 0.001. ( E ) Results from immunohistochemistry (IHC) showed that the expression of PDGF-AA ( yellow arrows ) was upregulated at MTrPs. Scale bars, 20 μm. Con, 1.51 ± 0.33; 95% CI, 1.16–1.85; versus MPS, 10.2 ± 1.57; 95% CI, 8.55–11.85; units, integrated optical density (IOD); mean ± SD; P < 0.001. ( F ) The cross-sectional area of muscle fibers was increased in MPS group. Con, 995.2 ± 166.5; 95% CI, 902.9–1,087; versus MPS, 1,398 ± 124.2; 95% CI, 1,330–1,467; units, μm 2 ; mean ± SD; P < 0.001. ( G ) Representative fluorescence microscopic images showing expression of p-PDGFR-α ( green ) in different groups. Scale bars, 20 μm. Con, 1.00 ± 0.10; 95% CI, 0.89–1.11; versus MPS, 1.44 ± 0.20; 95% CI, 1.23–1.64; units, mean intensity; mean ± SD; P < 0.001. * P < 0.05; ** P < 0.01; *** P < 0.001. ALK, anaplastic lymphoma kinase; EphA, ephrin receptor A; EphB, ephrin receptor B; LTK, leukocyte tyrosine kinase; TRKB, tyrosine kinase receptor B; ZAP70, zeta-chain-associated protein kinase 70.

Article Snippet: Blood samples from patients with MPS were tested for platelet-derived growth factor-AA (PDGF-AA) levels using a human PDGF-AA enzyme-linked immunosorbent assay (ELISA) kit (EK1696, Bosterbio, USA).

Techniques: Expressing, Derivative Assay, Control, Microarray, Microscopy, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Fluorescence